Prediction of the zeta potentials and ionic descriptors of a silica hydride stationary phase with mobile phases of different pH and ionic strength

• Zeta potential measurements used to study key surface properties of a silica hydride.
• Debye–Hückel theory used to describe changes in zeta potential and analyte retention.
• Origin of the retention behavior of basic, acidic and neutral analytes investigated.
• Linear solvation energy relationships reveal the role of ionic contributions.
• New insights into analyte retention mechanisms with ANPC methods garnered.

In this study, the zeta potentials of a silica hydride stationary phase (Diamond Hydride™) in the presence of different water–acetonitrile mixtures (from 0–80% (v/v) acetonitrile) of different ionic strengths (from 0–40 mM) and pH values (from pH 3.0–7.0) have been investigated. Debye–Hückel theory was applied to explain the effect of changes in the pH and ionic strength of these aqueous media on the negative zeta potential of this stationary phase. The experimental zeta potentials of the Diamond Hydride™ particles as a function of acetonitrile content up to 50% (v/v) correlated (R2 = 0.998) with the predicted zeta potential values based on this established theory, when the values of the dissociation constant of all related species, as well as viscosity, dielectric constant and refractive index of the aqueous medium were taken into consideration. Further, the retention behavior of basic, acidic and neutral analytes was investigated under mobile phase conditions of higher pH and lower ionic strength. Under these conditions, the Diamond Hydride™ stationary phase surface became more negative, as assessed from the increasingly more negative zeta potentials, resulting in the ion exchange characteristics becoming more dominant and the basic analytes showing increasing retention. Ionic descriptors were derived from these chromatographic experiments based on the assumption that linear solvation energy relationships prevail. The results were compared with predicted ionic descriptors based on the different calculated zeta potential values resulting in an overall correlation of R2 = 0.888. These studies provide fundamental insights into the impact on the separation performance of changes in the zeta potential of the Diamond Hydride™ surface with the results relevant to other silica hydride and, potentially, to other types of stationary phase materials.

Figure optionsDownload as PowerPoint slide