کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1164235 | 1491022 | 2014 | 5 صفحه PDF | دانلود رایگان |
• First automation of electrokinetically-pumped sheath–flow interface.
• Eight analyses of Escherichia coli digest performed in 8 h.
• An average of 1115 peptide IDs per run.
A PrinCE autosampler was coupled to a Q-Exactive mass spectrometer by an electrokinetically pumped sheath–flow nanospray interface to perform automated capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS). 20 ng aliquots of an Escherichia coli digest were injected to evaluate the system. Eight sequential injections over an 8-h period identified 1115 ± 70 (relative standard deviation, RSD = 6%) peptides and 270 ± 8 (RSD = 3%) proteins per run. The average RSDs of migration time, peak intensity, and peak area were 3%, 24% and 19%, respectively, for 340 peptides with high intensity. This is the first report of an automated CZE-ESI-MS/MS system using the electrokinetically pumped sheath–flow nanospray interface. The results demonstrate that this system is capable of reproducibly identifying over 1000 peptides from an E. coli tryptic digest in a 1-h analysis time.
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Journal: Analytica Chimica Acta - Volume 810, 31 January 2014, Pages 94–98