کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1164963 1491026 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel non-invasive electrochemical biosensing device for in situ determination of the alcohol content in blood by monitoring ethanol in sweat
ترجمه فارسی عنوان
دستگاه جدید بی حس کننده الکتروشیمیایی غیر تهاجمی برای تعیین محلول الکل در خون توسط نظارت بر اتانول در عرق
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• New electrochemical biosensing device for determining the blood's ethanol content (BAC).
• Prototype based on bienzyme amperometric composite biosensors.
• Determination of BAC by amperometric monitoring of ethanol in sweat.
• BAC determination in single measurement or in continuous modes.
• Successful validation with 40 volunteers.

A non-invasive, passive and simple to use skin surface based sensing device for determining the blood's ethanol content (BAC) by monitoring transdermal alcohol concentration (TAC) is designed and developed. The proposed prototype is based on bienzyme amperometric composite biosensors that are sensitive to the variation of ethanol concentration. The prototype correlates, through previous calibration set-up, the amperometric signal generated from ethanol in sweat with its content in blood in a short period of time. The characteristics of this sensor device permit determination of the ethanol concentration in isolated and in continuous form, giving information of the BAC of a subject either in a given moment or its evolution during long periods of time (8 h). Moreover, as the measurements are performed in a biological fluid, the evaluated individual is not able to alter the result of the analysis. The maximum limit of ethanol in blood allowed by legislation is included within the linear range of the device (0.0005–0.6 g L−1). Moreover, the device shows higher sensitivity than the breathalyzers marketed at the moment, allowing the monitoring of the ethanol content in blood to be obtained just 5 min after ingestion of the alcoholic drink. The comparison of the obtained results using the proposed device in the analysis of 40 volunteers with those provided by the gas chromatographic reference method for determination of BAC pointed out that there were no significant differences between both methods.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 806, 2 January 2014, Pages 1–7
نویسندگان
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