کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1165051 | 1491015 | 2014 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Label-free fluorescent biosensor based on the target recycling and Thioflavin T-induced quadruplex formation for short DNA species of c-erbB-2 detection Label-free fluorescent biosensor based on the target recycling and Thioflavin T-induced quadruplex formation for short DNA species of c-erbB-2 detection](/preview/png/1165051.png)
• The biosensor was based on target recycling and Thioflavin T-induced quadruplex.
• Thioflavin T was first used as a fluorescent indicator for biosensor fabrication.
• This sensor can detect as low as 20 fM DNA with ultra-high discrimination ability.
Non-invasive early diagnosis of breast cancer is the most effective way to improve the survival rate and increase more chances of breast-conserving. In this paper, we developed a label-free fluorescent biosensor based on nuclease assisted target recycling and Thioflavin T-induced quadruplex formation for short DNA species of c-erbB-2 detection in saliva. By employing the strategy, the sensor can detect as low as 20 fM target DNA with high discrimination ability even against single-base mismatch sequence. To the best of our knowledge, the proposed sensor is the first attempt to apply Thioflavin T that possesses outstanding structural selectivity for G-quadruplex in DNA amplification techniques, which may represent a promising path toward direct breast cancer detection in saliva at the point of care.
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Journal: Analytica Chimica Acta - Volume 817, 19 March 2014, Pages 42–47