کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1165822 1491092 2012 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High-performance liquid chromatography with fast-scanning fluorescence detection and multivariate curve resolution for the efficient determination of galantamine and its main metabolites in serum
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
High-performance liquid chromatography with fast-scanning fluorescence detection and multivariate curve resolution for the efficient determination of galantamine and its main metabolites in serum
چکیده انگلیسی

Based on green analytical chemistry principles, an efficient approach was applied for the simultaneous determination of galantamine, a widely used cholinesterase inhibitor for the treatment of Alzheimer's disease, and its major metabolites in serum samples. After a simple serum deproteinization step, second-order data were rapidly obtained (less than 6 min) with a chromatographic system operating in the isocratic regime using ammonium acetate/acetonitrile (94:6) as mobile phase. Detection was made with a fast-scanning spectrofluorimeter, which allowed the efficient collection of data to obtain matrices of fluorescence intensity as a function of retention time and emission wavelength. Successful resolution was achieved in the presence of matrix interferences in serum samples using multivariate curve resolution-alternating least-squares (MCR-ALS). The developed approach allows the quantification of the analytes at levels found in treated patients, without the need of applying either preconcentration or extraction steps. Limits of detection in the range between 8 and 11 ng mL−1, relative prediction errors from 7 to 12% and coefficients of variation from 4 to 7% were achieved.

Figure optionsDownload as PowerPoint slideHighlights
► A simple and direct analytical method for galantamine and its serum metabolites is developed.
► Low detection limits are rapidly achieved, with minimal organic reagents and sample amounts.
► Quantification is accomplished using green-chemistry principles.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 740, 31 August 2012, Pages 27–35
نویسندگان
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