Separation of carboxylic acids in human serum by isotachophoresis using a commercial field-deployable analytical platform combined with in-house glass microfluidic chips

► A glass ITP microfluidic chip was designed for analysis of lactate in human serum. ► ITP chip was compatible with a field-deployable commercial platform. ► LIF/LEDIF indirect fluorescence detection was used. ► Lack of research-flexible commercial microfluidic separation platform is addressed.