کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1166083 1491103 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Dual lifetime referenced fluorometry for the determination of doxorubicin in urine
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Dual lifetime referenced fluorometry for the determination of doxorubicin in urine
چکیده انگلیسی

Dual lifetime referencing (DLR) is introduced as a rapid and self-referenced method for measuring the concentration of a fluorescent analyte in solution. The fluorescent cancer chemotherapeutic doxorubicin was chosen as a medically relevant analyte and blended with a reference dye (Ru(dpp)3) that displays overlapping excitation and emission spectra. The relative contributions of the short-lived (nanoseconds) fluorescent analyte and the long-lived (microseconds) reference dye define the observed lifetime. Measuring this lifetime by both frequency-domain DLR and time-domain DLR yields similar analytical ranges and limits of detection (0.4 μM). To assess the matrix effect of medical samples, the standard addition method was employed to both modes of DLR. Urine was spiked with doxorubicin and recovery rates of ≥97% were obtained.

Figure optionsDownload as PowerPoint slideHighlights
► Self-referenced determination of fluorescent analytes in solution by dual lifetime referencing.
► Comparison of frequency-domain DLR and time-domain DLR.
► DLR has a detection limit of 400 nM doxorubicin.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 729, 4 June 2012, Pages 62–66
نویسندگان
, , ,