Preparation, characterization and application of organic–inorganic hybrid ractopamine multi-template molecularly imprinted capillary monolithic column

Four isomers of ractopamine used as multi-template molecule, a molecularly imprinted capillary monolithic column was prepared by organic–inorganic hybrid method. The prepared imprinting capillary monolithic columns were characterized by SEM and FTIR, evaluated under capillary electrochromatograhy and the effect of electroosmotic flow and selectivity were studied in detail. It was found that the prepared imprinted monolithic column had good flow-through property when the ratio of methanol and toluene was 3:2. The result indicated that the imprinted monolithic column has good selectivity for four isomers of ractopamine, and under the conditions of pH value of buffer solution was 7 and 10% acetonitrile content in buffer, four isomers of ractopamine could be separated completely.