Development of a combined technique using a rapid one-step immunochromatographic assay and indirect competitive ELISA for the rapid detection of baicalin

- We developed a combined immunoassay using an immunochromatographic assay (ICA) and indirect competitive ELISA (icELISA) using anti-baicalin monoclonal antibody (anti-BA MAb).
- The ICA enables a short assay time (15 min), no dependence on any instrumental systems.
- In addition, the icELISA is suitable for quantitative analysis of BA and baicalein with reliability and accuracy.
- The developed immunochemical system can provide practical and efficient applications toward monitoring the quality of Scutellariae Radix and ensure proper use of Kampo medicines containing S. Radix.