کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1166768 | 1491109 | 2012 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Electrochemical detection of a powerful estrogenic endocrine disruptor: Ethinylestradiol in water samples through bioseparation procedure Electrochemical detection of a powerful estrogenic endocrine disruptor: Ethinylestradiol in water samples through bioseparation procedure](/preview/png/1166768.png)
The synthetic estrogen ethinylestradiol (EE2) is an active component of oral contraceptives (OCs), considered as an endocrine disrupting compound (EDC). It is excreted from humans and released via sewage treatment plant effluents into aquatic environments. EDCs are any environmental pollutant chemical that, once incorporated into an organism, affects the hormonal balance of various species including humans. Its presence in the environment is becoming of great importance in water quality. This paper describes the development of an accurate, sensitive and selective method for capture, preconcentration and determination of EE2 present in water samples using: magnetic particles (MPs) as bioaffinity support for the capture and preconcentration of EE2 and a glassy carbon electrode modified with multi-walled carbon nanotubes (MWCNTs/GCE) as detection system. The capture procedure was based on the principle of immunoaffinity, the EE2 being extracted from the sample using the anti-EE2 antibodies (anti-EE2 Ab) which were previously immobilized on MPs. Subsequently the analyte desorption was done employing a sulfuric acid solution and the determination of the EE2 in the pre-concentrated solution was carried out by square wave voltammetry (SWV).This method can be used to determine EE2 in the range of 0.035–70 ng L−1 with a detection limit (LOD) of 0.01 ng L−1 and R.S.D. < 4.20%. The proposed method has been successfully applied to the determination of EE2 in water samples and it has promising analytical applications for the direct determination of EE2 at trace levels.
Figure optionsDownload as PowerPoint slideHighlights
► We developed an electrochemical method for the determination of ethinylestradiol in water samples.
► Modified magnetic particles were employed for the bioseparation and preconcentration procedures.
► The use of magnetic particles offers high selectivity due to antigen–antibody binding specificity.
► The detection was carried out by using a glassy carbon electrode modified with carbon nanotubes.
Journal: Analytica Chimica Acta - Volume 723, 20 April 2012, Pages 27–32