کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1167085 | 1491123 | 2012 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Rational design and synthesis of water-compatible molecularly imprinted polymers for selective solid phase extraction of amiodarone Rational design and synthesis of water-compatible molecularly imprinted polymers for selective solid phase extraction of amiodarone](/preview/png/1167085.png)
Novel water-compatible molecularly imprinted polymers (MIPs) selective for amiodarone (AD) were designed via a new methodology which relies on screening library of non-imprinted polymers (NIPs). The NIP library consisted of eighteen cross-linked co-polymers synthesized from monomers commonly used in molecular imprinting. The binding capacity of each polymer in the library was analyzed in two different solvents. Binding in water was used to assess non-specific (hydrophobic) interactions and binding in an appropriate organic solvent was used to assess specific interactions. A good correlation was found between the screening tests and modeling of monomer–template interactions performed using computational approach. Additionally, analysis of template–monomer interactions was performed using UV–vis spectroscopy. As the result, 4-vinylpyridine (4-VP) was selected as the best monomer for developing MIP for AD. The 4-VP-based polymers demonstrated imprinting factor equal 3.9. The polymers performance in SPE was evaluated using AD and its structural analogues. The recovery of AD was as high as 96% when extracted from spiked phosphate buffer (pH 4.5) solution and 82.1% from spiked serum samples. The developed MIP shown as a material with specific binding to AD, comparing to its structural analogues, 1-(2-diethylaminoethoxy)-2,6-diiodo-4-nitrobenzene and lidocaine, which shown 9.9% and 25.4% of recovery from the buffer solution, correspondingly. We believe that the screening of NIP library could be proposed as an alternative to commonly used computational and combinatorial approaches.
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► We developed a non-imprinted polymer (NIP) library with 18 monomers.
► Monomers were screened against amiodarone with NIP library by binding test.
► NIP library screening had similar result with computer modeling.
► High selective water compatible imprinted polymers were made with the monomer selected by the screening.
► The polymers were used in solid-phase extraction of amiodarone from aqueous sample.
Journal: Analytica Chimica Acta - Volume 709, 4 January 2012, Pages 98–104