Description and validation of coupling high performance liquid chromatography with resonance Rayleigh scattering in aminoglycosides determination

In view of the fact that many substances generally exhibit very little ultraviolet absorbance and the absence of native fluorescence, a new strategy with simple instrumentation and excellent analytical performance combining high performance liquid chromatography (HPLC) with resonance Rayleigh scattering (RRS) was developed. It was validated for the quantification of aminoglycosides (AGs). This fact was also carefully calculated by quantum chemistry. However, the sensitivity was probably limited by the volume of flow-through cell. Therefore, the result calls for a suitable one to ensure optimal RRS signal. Interestingly, when serum or urine samples of analytes were analyzed by this method, they were all well resolved without any interference, which would hold a new perspective to be applied in the determination of substances in biological matrix.

Theoretical and experimental analysis had proved that aminoglycosides reacted with Congo red to form binary compounds simultaneously, which led to a novel HPLC–RRS strategy being applied in substances which are not fluorescing and not UV absorbed.Figure optionsDownload as PowerPoint slideHighlights
► A novel HPLC–RRS strategy was shown in this study.
► Theoretical and experimental analysis had proved the feasibility of this method.
► Because of its specificity, no interference from the matrix was observed.
► The analytes in biological matrix were all well resolved without any interference.
► It provided new insights for analytes lack of useful spectroscopic and electrochemical properties.