کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1167345 960538 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sensitive and simple detection of Escherichia coli strain based on time-resolved fluorescence DNA hybridization assay
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Sensitive and simple detection of Escherichia coli strain based on time-resolved fluorescence DNA hybridization assay
چکیده انگلیسی

A two-probe tandem DNA hybridization assay based on time-resolved fluorescence was employed to detect Escherichia coli strain. The amino modified capture probe was covalently immobilized on the common glass slide surface. The Eu(TTA)3(5-NH2-phen) with the characteristics of long lifetime and intense luminescence was labeled with reporter probe. The original extracted DNA samples without the purification and amplification process were directly used in the hybridization assay. The concentration of capture probe, hybridization temperature, hybridization and washing time were optimized. The detection limit is about 1.49 × 103 CFU mL−1E. coli cells, which is comparable to the value of most microbiology methods. The proposed method has the advantages of easy operation, satisfactory sensitivity and specificity, which can provide a promising technique for monitoring the microorganisms.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 664, Issue 1, 1 April 2010, Pages 95–99
نویسندگان
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