کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1168044 | 1491163 | 2009 | 7 صفحه PDF | دانلود رایگان |
Multivariate deconvolution methods applied for resolution of overlapping chromatographic peaks require bilinearity. This implies that the shape of the spectrum of each analyte should not change during the elution of a compound. However, in chromatography, the concentration of a compound in the detector changes rapidly. For scanning instruments, like most mass spectrometers where the ions within a scan are not recorded simultaneously, these concentration differences introduce significant differences between spectra on the left and right side of the chromatographic peak. Because of this scan-effect, the assumption of bilinearity is not valid. Several methods, all having their limitations, have previously been applied to reduce this effect. In this paper a methodology for reduction of the scan-effect by a rank annihilation approach is described, and applications on both simulated and experimental data are shown. The rank annihilation approach is more efficient and has a broader range of applications than previously described methods.
Journal: Analytica Chimica Acta - Volume 640, Issues 1–2, 27 April 2009, Pages 33–39