کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1168216 960580 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A new matrix for analyzing low molecular mass compounds and its application for determination of carcinogenic areca alkaloids by matrix-assisted laser desorption ionization time-of-flight mass spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A new matrix for analyzing low molecular mass compounds and its application for determination of carcinogenic areca alkaloids by matrix-assisted laser desorption ionization time-of-flight mass spectrometry
چکیده انگلیسی

Arecoline is the main alkaloid present in the areca nut (or betel nut) and it has central nervous system effects. Its pharmacological activities induce the constriction of the bronchial smooth muscles, and stimulation of the lacrimal and intestinal glands. Chewing areca nut is harmful to health because this habit may increase the risk of the development of oral cancer. In this study, a fast method was provided for the determination of areca alkaloids by matrix-assisted laser desorption ionization (MALDI) mass spectrometer with a time-of-flight (TOF) analyzer. Traditionally the MALDI-TOF method was not suitable for the analysis of small molecular weight (m/z < 600) compounds because of the high background of the matrix. In this study, a new matrix was utilized to decrease the background interference effectively. After simple sample preparation, 1 μL sample supernatant was mixed with 1 μL matrix and then deposited on the target plate. This new matrix was also used to test the MALDI imaging experiment. Application of this MALDI-TOF method for trace analysis of arecoline by this new matrix in human plasma at sub μM level proved workable.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 649, Issue 2, 7 September 2009, Pages 230–235
نویسندگان
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