کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1168917 | 960611 | 2009 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Selective spectrofluorimetric determination of glutathione in clinical and biological samples using 1,3,5,7-tetramethyl-8-phenyl-(2-maleimide)-difluoroboradiaza-s-indacene Selective spectrofluorimetric determination of glutathione in clinical and biological samples using 1,3,5,7-tetramethyl-8-phenyl-(2-maleimide)-difluoroboradiaza-s-indacene](/preview/png/1168917.png)
This work reports the development of a selective, sensitive and rapid spectrofluorimetric method for the determination of reduced glutathione (GSH) in the presence of relatively high levels of cysteine (Cys) in clinical and biological samples using 1,3,5,7-tetramethyl-8-phenyl-(2-maleimide)-difluoroboradiaza-s-indacene (TMPAB-o-M). The fluorescence from TMPAB-o-M is strongly quenched by its maleimide moiety, but after reaction with thiol, the fluorescence is restored with a 350-fold intensity increase (fluorescence quantum yield from 0.002 to 0.73). In H3Cit–Na2HPO4 buffer (pH 7.40), the derivatization is completed in just 5 min under 37 °C. The linear range is 0.005–0.2 μmol L−1, with detection limit of 1.1 × 10−10 mol L−1 (signal-to-noise ratio = 3). Almost all amino acids, including Cys, impose no interference even if present at relatively high concentrations (amino acids:GSH = 100:1, Cys:GSH = 1:1, molar ratio, CGSH = 3 × 10−7 mol L−1). The sample can be used directly without further treatment after the protein is removed. The developed method is precise with a relative standard deviation (R.S.D.) lower than 5.0% (n = 6) and has been applied to the determination of GSH in human blood and pig’s liver with recoveries between 94.4 and 105.6%.
Journal: Analytica Chimica Acta - Volume 633, Issue 1, 2 February 2009, Pages 71–75