کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1169622 960643 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A reagentless amperometric immunosensor for α-1-fetoprotein based on gold nanowires and ZnO nanorods modified electrode
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A reagentless amperometric immunosensor for α-1-fetoprotein based on gold nanowires and ZnO nanorods modified electrode
چکیده انگلیسی

A novel strategy for the preparation of reagentless immunosensor for rapid determination of α-1-fetoprotein (AFP) in human serum has been developed. The immunosensor was prepared by immobilizing α-1-fetoprotein antibody (AFP Ab) onto the glassy carbon electrode modified by gold nanowires (Au NWs) and ZnO nanorods (ZnO NRs) composite film. Gold nanowires and ZnO nanorods were produced by an electrodeposition strategy using nanopore polycarbonate (PC) membrane. A sandwich immunoassay format was employed to detect AFP with horseradish peroxidase (HRP)-labeled AFP as tracer. The morphology of the Au NWs and ZnO NRs composite film has been investigated by scanning electron microscopy (SEM) and energy disperse spectroscopy (EDS) analysis. The resulting immunosensor offered an excellent amperometric response for AFP ranging from 0.5 to 160.0 ng mL−1 with a detection limit of 0.1 ng mL−1. Because of the combination of the biocompatibility of ZnO NRs and the direct electron-transfer of Au NWs between HRP and electrode, the proposed immunosensor displayed a direct electrochemical response of HRP to the reduction of H2O2 with high sensitivity, quick response, good repeatability and long-term stability.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 615, Issue 2, 19 May 2008, Pages 158–164
نویسندگان
, , , , , , ,