کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1169710 960647 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Light scattering sensing detection of pathogens based on the molecular recognition of immunoglobulin with cell wall-associated protein A
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Light scattering sensing detection of pathogens based on the molecular recognition of immunoglobulin with cell wall-associated protein A
چکیده انگلیسی

In this contribution, we report a rapid optical detection method of pathogens using Staphylococcus aureus (S. aureus) as the model analyte based on the molecular recognition of immunoglobulin with cell wall-associated Protein A (SpA). It was found that the molecular recognition of human immunoglobulin (IgG) with protein A on the cell wall of S. aureus on glass slide sensing area could result in strong surface enhanced light scattering (SELS) signals, and the SELS intensity (ΔI) increases proportionally with the concentration of S. aureus over the range of 2.5 × 105–1.0 × 108 CFU mL−1 with right angle light scattering (RALS) signals detection mode. In order to identify the solid support based molecular recognition between IgG with SpA, we also employed water-soluble CdS quantum dots (CdS-QDs) as a fluorescent marker for IgG by immobilizing the IgG onto the surfaces of CdS-QDs through covalent binding in order to generate recognition probes for SpA on the cell wall of S. aureus. Consequently, the fluorescent method also showed that the detection for pathogens with solid supports is reliable based on the molecular recognition of IgG with SpA.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 599, Issue 2, 19 September 2007, Pages 279–286
نویسندگان
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