کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1169966 | 960659 | 2008 | 7 صفحه PDF | دانلود رایگان |

In this article, we present a systematic study on IgG and Fab fragment of anti-IgG molecules using fluorescence auto- and cross-correlation spectroscopy to investigate their diffusion characteristics, binding kinetics, and the effect of small organic molecule, urea on their binding. Through our analysis, we found that the diffusion coefficient for IgG and Fab fragment of anti-IgG molecules were 37 ± 2 μm2 s−1 and 56 ± 2 μm2 s−1, respectively. From the binding kinetics study, the respective forward (ka) and backward (kd) reaction rates were (5.25 ± 0.25) × 106 M−1 s−1 and 0.08 ± 0.005 s−1, respectively and the corresponding dissociation binding constant (KD) was 15 ± 2 nM. We also found that urea inhibits the binding of these molecules at 4 M concentration due to denaturation.
Journal: Analytica Chimica Acta - Volume 625, Issue 1, 5 September 2008, Pages 103–109