کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1170391 960678 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantification of protein based on single-molecule counting by total internal reflection fluorescence microscopy with adsorption equilibrium
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Quantification of protein based on single-molecule counting by total internal reflection fluorescence microscopy with adsorption equilibrium
چکیده انگلیسی
We developed a sensitive single-molecule imaging method for quantification of protein by total internal reflection fluorescence microscopy with adsorption equilibrium. In this method, the adsorption equilibrium of protein was achieved between solution and glass substrate. Then, fluorescence images of protein molecules in a evanescent wave field were taken by a highly sensitive electron multiplying charge coupled device. Finally, the number of fluorescent spots corresponding to the protein molecules in the images was counted. Alexa Fluor 488-labeled goat anti-rat IgG(H + L) was chosen as the model protein. The spot number showed an excellent linear relationship with protein concentration. The concentration linear range was 5.4 × 10−11 to 8.1 × 10−10 mol L−1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 590, Issue 1, 2 May 2007, Pages 104-109
نویسندگان
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