کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1170484 | 960681 | 2007 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Tracking of mercury ions in living cells with a fluorescent chemodosimeter under single- or two-photon excitation Tracking of mercury ions in living cells with a fluorescent chemodosimeter under single- or two-photon excitation](/preview/png/1170484.png)
Tracking of Hg2+ in solutions as well as in living cells was conducted with a fluorescent chemodosimeter by measuring the spectral shift of its fluorescence under single- or two-photon excitation. The spectral hypsochromic shifts of this chemodosimeter when reacting with Hg2+ were found to be about 50 nm in acetonitrile/water solutions and 32 nm in Euglena gracilis 277 living cells. This chemodosimeter shows high sensitivity and selectivity, and is not influenced by the pH values. It can signal Hg2+ in solutions down to the ppb range under either single-photon excitation (SPE) at 405 nm or two-photon excitation (TPE) at 800 nm. However, with low cellular chemodosimeter concentrations, the SPE spectra were disturbed by the auto-fluorescence from the native fluorophore in the cell, while the TPE spectra were still of high quality since the two-photon absorption cross section of this chemodosimeter is much larger than that of the native fluorophores in the cell.
Journal: Analytica Chimica Acta - Volume 597, Issue 2, 10 August 2007, Pages 306–312