کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1170972 960698 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enzyme immunoassay for semicarbazide—The nitrofuran metabolite and food contaminant
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Enzyme immunoassay for semicarbazide—The nitrofuran metabolite and food contaminant
چکیده انگلیسی

Semicarbazide (SEM), the marker residue for the banned nitrofuran veterinary antibiotic nitrofurazone (NFZ), has been detected regularly in foods (47% of recent nitrofuran EU Rapid Alerts involve SEM). However, the validity of SEM as a definitive marker for NFZ has been undermined by SEM arising from other sources including azodicarbonamide, a plastics blowing agent and flour treatment additive. An inexpensive screening test for SEM in food matrices is needed—all SEM testing currently uses expensive LC–MS/MS instrumentation. We now report the first production of antibodies against derivatised SEM. A novel carboxyphenyl SEM derivative was used to raise a polyclonal antibody that has been incorporated into a semi-quantitative microtitre plate ELISA, validated according to the criteria set out in Commission Decision 2002/657/EC, for use with chicken muscle. The antibody is highly specific for derivatised SEM, cross-reactivity being 1.7% with NFZ and negligible with a wide range of other nitrofurans and poultry drugs. Samples are derivatised with o-nitrobenzaldehyde and simultaneously protease digested before extraction by cation exchange SPE. The ELISA has a SEM detection capability (CCβ) of 0.25 μg kg−1 when a threshold of 0.21 μg kg−1 is applied to the selection of samples for confirmation (lowest observed 0.25 μg kg−1 fortified sample, n = 20), thus satisfying the EU nitrofurans’ minimum required performance limit of 1 μg kg−1. NFZ-incurred muscles (12) containing SEM at 0.5–5.0 μg kg−1 by LC–MS/MS, all screened positive by this ELISA protocol which is also applicable to egg and chicken liver.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 592, Issue 1, 29 May 2007, Pages 64–71
نویسندگان
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