کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1171132 960706 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fast determination of paeonol in plasma by headspace solid-phase microextraction followed by gas chromatography-mass spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Fast determination of paeonol in plasma by headspace solid-phase microextraction followed by gas chromatography-mass spectrometry
چکیده انگلیسی
Paeonol is the active component in the traditional Chinese medicines (TCMs), such as Cynanchum paniculatum, which has been used to treat many diseases, such as eczema. In this work, a simple, rapid and sensitive method was developed for the determination of paeonol in rabbit plasma, which was based on headspace solid-phase microextraction (HS-SPME) followed by gas chromatography-mass spectrometry (GC-MS). The extraction parameters of fiber coating, sample temperature, extraction time, stirring rate and ion strength were systemically optimized; the method linearity, detection limit and precision were also investigated. It was shown that the proposed method provided a good linearity (0.02-20 μg mL−1, R2 > 0.990), low detection limit (2.0 ng mL−1) and good precision (R.S.D. value less than 8%). Finally, GC/MS following HS-SPME was applied to fast determination of paeonol in rabbit plasma at different time point after oral demonstration of Cynanchum paniculatum essential oil. The experimental results suggest that the proposed method provided an alternative and novel approach to the pharmacokinetics study of paeonol in the TCMs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 585, Issue 1, 28 February 2007, Pages 76-80
نویسندگان
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