کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1172182 | 1491182 | 2006 | 9 صفحه PDF | دانلود رایگان |

A fully-automated fluidic system for a bead-based immunoassay with electrochemical detection was developed. Assays for the bacteriophage MS2 and ovalbumin (OVA) were demonstrated using this system. Streptavidin-coated paramagnetic microbeads were used as a mobile solid phase. The immunoassay sandwich was made by attaching a biotinylated antibody to the streptavidin-coated beads, capturing antigen, and then exposing the antigen to an antibody conjugated with β-galactosidase. β-Galactosidase converts p-aminophenyl galactopyranoside (PAPG) to p-aminophenol (PAP), which is electrochemically oxidized to p-quinone imine (PQI). The behavior of paramagnetic microbeads in the fluidic system was investigated using beads with immobilized β-galactosidase at different concentrations of beads and flow rates for each procedure in the assay. Furthermore, the fully-automated MS2 and OVA assays were demonstrated using the fluidic system. The limits of detection for MS2 and OVA were 990 (1.6 × 1011 particles mL−1) and 470 ng mL−1, respectively.
Journal: Analytica Chimica Acta - Volume 561, Issues 1–2, 2 March 2006, Pages 69–77