کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1172326 | 1491185 | 2006 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Application of carbon nanotube-matrix assistant native polyacrylamide gel electrophoresis to the separation of apolipoprotein A-I and complement C3
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Application of carbon nanotube-matrix assistant native polyacrylamide gel electrophoresis to the separation of apolipoprotein A-I and complement C3 Application of carbon nanotube-matrix assistant native polyacrylamide gel electrophoresis to the separation of apolipoprotein A-I and complement C3](/preview/png/1172326.png)
چکیده انگلیسی
The application of carbon nanotube polyacrylamide gel electrophoresis (PAGE) is being reported so as to improve selectivity of analysis. A novel polyacrylamide gel was manufactured by adding multi-walled carbon nanotubes (MWNT) when producing the polyacrylamide gel. Carbon nanotube-matrix assistance was used to separate apolipoprotein A-I and complement C3 with native polyacrylamide gel electrophoresis. Significant improvement of the relative selectivity was achieved in the separation between apolipoprotein A-I and complement C3, because of the hydrophobic change of the carbon nanotube surface non-covalently functionalized by the surfactant Triton X-100. These two proteins were identified by MS/MS after electrophoresis. The change of the observed mobility can also be noticed using other nanomaterials such as nano-TiO2 and nano-Al2O3, but no significant improvement of relative selectivities could be observed with the latter nanoparticles.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 557, Issues 1â2, 31 January 2006, Pages 137-145
Journal: Analytica Chimica Acta - Volume 557, Issues 1â2, 31 January 2006, Pages 137-145
نویسندگان
Guangming Huang, Yangjun Zhang, Jin Ouyang, Willy R.G. Baeyens, Joris R. Delanghe,