کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1172573 960791 2006 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Designing split reporter proteins for analytical tools
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Designing split reporter proteins for analytical tools
چکیده انگلیسی

A current focus of biological research is to quantify and image cellular processes in living cells and animals. To detect such cellular processes, genetically-encoded reporters have been extensively used. The most common reporters include firefly luciferase, renilla luciferase, green fluorescent protein (GFP) and its variants with various spectral properties. This review describes novel design of split-GFP and luciferase reporters based on protein splicing, and highlights some potential applications with the reporters to study protein–protein interactions, protein localization, intracellular protein dynamics, and protein activity in living cells and animals.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 556, Issue 1, 18 January 2006, Pages 58–68
نویسندگان
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