کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1172573 | 960791 | 2006 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Designing split reporter proteins for analytical tools
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A current focus of biological research is to quantify and image cellular processes in living cells and animals. To detect such cellular processes, genetically-encoded reporters have been extensively used. The most common reporters include firefly luciferase, renilla luciferase, green fluorescent protein (GFP) and its variants with various spectral properties. This review describes novel design of split-GFP and luciferase reporters based on protein splicing, and highlights some potential applications with the reporters to study protein–protein interactions, protein localization, intracellular protein dynamics, and protein activity in living cells and animals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 556, Issue 1, 18 January 2006, Pages 58–68
Journal: Analytica Chimica Acta - Volume 556, Issue 1, 18 January 2006, Pages 58–68
نویسندگان
Takeaki Ozawa,