کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1177719 | 962566 | 2016 | 7 صفحه PDF | دانلود رایگان |
• A SILAC-based quantitative proteomic study of TBMS1's anti-tumor cytotoxicity
• 29 ribosomal proteins were differentially up-regulated by TBMS1 in NCI-H460 cells.
• TBMS1 treatment induced nucleolar stress of NCI-H460 and activated p53/MDM2, mTOR and NF-κB signaling pathways.
Tubeimoside-1 (TBMS1) exerts its anticancer effects by inducing G2/M arrest and apoptosis of cancer cells. However, the precise molecular mechanism of its anti-tumor effects has not been fully elucidated, especially the signaling pathways involved in the early stage of TBMS1 stimulation. In this study, we employed stable isotope labeling by amino acids in cell culture (SILAC)-based quantitative proteomics approach and identified 439 proteins that exhibit significant differential expressions in NCI-H460 lung cancer cells upon exposure to TBMS1. Gene ontology and network analysis using DAVID and STRING on-line tools revealed that several nucleolar stress (ribosomal biogenesis) response proteins were differentially regulated by TBMS1. Functional validation demonstrated that TBMS1-induced NCI-H460 cell cytotoxicity involved nucleolar stress-induced p53/murine double minute clone 2 (MDM2), mTOR, and NF-κB signaling pathways.
Figure optionsDownload high-quality image (317 K)Download as PowerPoint slide
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1864, Issue 2, February 2016, Pages 204–210