کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1178241 | 1491454 | 2008 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Proteomic profiling of endothelin-1-stimulated hypertrophic cardiomyocytes reveals the increase of four different desmin species and α-B-crystallin
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کلمات کلیدی
TFAEt1MitoTrackerCRYABDRMIEFSfMTBSTCAACNPTMIFSDCM2DE - 2deIPG - GPAimmobilized pH gradient - gradient pH immobilizedAcetonitrile - استونیتریلTrifluoroacetic acid - اسید Trifluoroacetictrichloroacetic acid - اسید ترشکلراکتیکpost-translational modification - اصلاح post-translational2-Dimensional gel electrophoresis - الکتروفورز ژل دو بعدیendothelin - اندوتلینendothelin-1 - اندوتلین-1Tris-buffered saline - تریس بافر شورisoelectric focusing - تمرکز ذره ای الکتریکیDesmin - دسمنIntermediate filaments - رشته های متوسطMALDI-TOF-MS - مالدی TOF-MSSerum-free medium - محیط بدون سرمMitochondria - میتوکندریاHypertrophy - هیپرتروفی یا پُرسازیProteomics - پروتئومیکسDilated cardiomyopathy - کاردیومیوپاتی دیلاته، کاردیومیوپاتی کامل
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We performed a proteomic investigation on primary cultures of neonatal rat cardiomyocytes after treatment with 10 nM endothelin-1 (ET1) for 48 h, an in vitro model for cardiac hypertrophy. Two-dimensional gel electrophoresis profiles of cell lysates were compared after colloidal Coomassie Blue staining. 12 protein spots that significantly changed in density due to ET1 stimulation were selected for in-gel digestion and identified through mass spectrometry. Of these, 8 spots were increased and 4 were decreased. Four of the increased proteins were identified as desmin, the cardiac component of intermediate filaments and one as α-B-crystallin, a molecular chaperone that binds desmin. All the desmins increased 2- to 5-fold, and α-B-crystallin increased 2-fold after ET1 treatment. Desmin cytoskeleton has been implicated in the regulation of mitochondrial activity and distribution, as well as in the formation of amyloid bodies. Mitochondria-specific fluorescent probe MitoTracker indicated mitochondrial redistribution in hypertrophic cells. An increase of amyloid aggregates containing desmin upon treatment with ET1 was detected by filter assay. Of the four proteins that showed decreased abundance after ET1 treatment, the chaperones hsp60 and grp75 were decreased 13- and 9-fold, respectively. In conclusion, proteomic profiling of ET1-stimulated rat neonatal cardiomyocytes reveals specific changes in cardiac molecular phenotype mainly involving intermediate filament and molecular chaperone proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1784, Issues 7â8, JulyâAugust 2008, Pages 1068-1076
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1784, Issues 7â8, JulyâAugust 2008, Pages 1068-1076
نویسندگان
Giulio Agnetti, Karel Bezstarosti, Dick H.W. Dekkers, Adrie J.M. Verhoeven, Emanuele Giordano, Carlo Guarnieri, Claudio M. Caldarera, Jennifer E. Van Eyk, Jos M.J. Lamers,