کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1178461 962694 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Secretome analysis using a hollow fiber culture system for cancer biomarker discovery
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Secretome analysis using a hollow fiber culture system for cancer biomarker discovery
چکیده انگلیسی

Secreted proteins, collectively referred to as the secretome, were suggested as valuable biomarkers in disease diagnosis and prognosis. However, some secreted proteins from cell cultures are difficult to detect because of their intrinsically low abundance; they are frequently masked by the released proteins from lysed cells and the substantial amounts of serum proteins used in culture medium. The hollow fiber culture (HFC) system is a commercially available system composed of small fibers sealed in a cartridge shell; cells grow on the outside of the fiber. Recently, because this system can help cells grow at a high density, it has been developed and applied in a novel analytical platform for cell secretome collection in cancer biomarker discovery. This article focuses on the advantages of the HFC system, including the effectiveness of the system for collection of secretomes, and reviews the process of cell secretome collection by the HFC system and proteomic approaches to discover cancer biomarkers. The HFC system not only provides a high-density three-dimensional (3D) cell culture system to mimic tumor growth conditions in vivo but can also accommodate numerous cells in a small volume, allowing secreted proteins to be accumulated and concentrated. In addition, cell lysis rates can be greatly reduced, decreasing the amount of contamination by abundant cytosolic proteins from lysed cells. Therefore, the HFC system is useful for preparing a wide range of proteins from cell secretomes and provides an effective method for collecting higher amounts of secreted proteins from cancer cells. This article is part of a Special Issue entitled: An Updated Secretome.

Figure optionsDownload high-quality image (122 K)Download as PowerPoint slideHighlights
► The secreted proteins are easily masked by other abundant proteins.
► Enriching secretome collection is an important step for secretomic analysis.
► The hollow fiber culture (HFC) system is developed for cancer biomarker discovery.
► This system can reduce cell lysis and provide a 3D culture environment.
► The HFC system provides an effective method to enrich secretome.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1834, Issue 11, November 2013, Pages 2285–2292
نویسندگان
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