کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1178887 | 962739 | 2010 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantitative proteomic analysis of ribosomal protein L35b mutant of Saccharomyces cerevisiae
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کلمات کلیدی
PBSSBDSSGA2-DEGFPsynthetic genetic array - آرایه مصنوعی ژنتیکیtwo-dimensional gel electrophoresis - الکتروفورز ژل دو بعدیTandem affinity purification - تصفیه وابستگی دو طرفهreverse transcription - رونویسی معکوسSaccharomyces cerevisiae - ساکارومایسس سرویزیهTAP - ضربه زدنPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریGene ontology - هستیشناسی ژنیProteome - پروتئومribosomal protein - پروتئین ریبوزومیgreen fluorescent protein - پروتئین فلورسنت سبزoptical density - چگالی نوریsynthetic complete - کامل مصنوعی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Recent studies have revealed that in higher eukaryotes, several ribosomal proteins are involved in some pathological events or developmental defects, indicating that ribosomal proteins perform unconventional functions other than protein biosynthesis. To obtain an insight into the novel roles of ribosomal proteins, we aimed to analyze the changes in proteome expression in ribosomal protein mutants by using Saccharomyces cerevisiae as a model system. We introduced the rpl35bÎ mutation into the 4159 green fluorescent protein (GFP)-tagged yeast strains by using the synthetic genetic array (SGA) method, and performed quantitative proteomic analysis by using a multilabel microplate reader and flow cytometer. We identified 22 upregulated and 20 downregulated proteins in the rpl35bÎ mutant. These proteins were primarily classified into the Gene Ontology (GO) categories of cellular biosynthetic process, translation, protein or nucleotide metabolic process, cell wall organization and biogenesis, and hyperosmotic response. We also investigated the correlation between the mRNA and protein levels of the identified proteins. Our results show that a ribosomal protein mutation can lead to perturbation in the expression of several proteins, including some other ribosomal proteins. Furthermore, our approach of combining a library of GFP-tagged yeast strains and the SGA method provides an effective and highly sensitive method for dynamic analysis of the effects of various mutations on proteome expression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1804, Issue 4, April 2010, Pages 676-683
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1804, Issue 4, April 2010, Pages 676-683
نویسندگان
Yong Bhum Song, Min A Jhun, Taesung Park, Won-Ki Huh,