کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1179828 962801 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Probes of the mitochondrial cAMP-dependent protein kinase
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Probes of the mitochondrial cAMP-dependent protein kinase
چکیده انگلیسی

The development of a fluorescent assay to detect activity of the mitochondrial cAMP-dependent protein kinase (PKA) is described. A peptide-based sensor was utilized to quantify the relative amount of PKA activity present in each compartment of the mitochondria (the outer membrane, the intermembrane space, and the matrix). In the process of validating this assay, we discovered that PKA activity is regulated by the protease calpain. Upon exposure of bovine heart mitochondria to digitonin, Ca2 +, and a variety of electron transport chain inhibitors, the regulatory subunits of the PKA holoenzyme (R2C2) are digested, releasing active catalytic subunits. This proteolysis is attenuated by calpain inhibitor I (ALLN). This article is part of a Special Issue entitled: Inhibitors of Protein Kinases (2012).


► Development of a fluorescent sensor for PKA with a large dynamic range
► Determination of the suborganelle location of mitochondrial PKA activity
► Discovery of a proteolytic regulation of mitochondrial PKA activity

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1834, Issue 7, July 2013, Pages 1359–1363
نویسندگان
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