Progress on Molecular Mechanism of Phosphorylation/Dephosphorylation and Detection Technology of γH2AX

Since γH2AX was firstly found in 1998, it has been one of the most important scientific topics and research tools in the related scientific fields. At present, a series of advanced analytical techniques were developed, which exhibited a quite attractive application prospect in the area of life science and medical science. This paper reviews the latest progress about γH2AX in terms of molecular mechanism of phosphorylation/dephosphorylation, the development of testing techniques, and the related applications.

As a novel biomarker of DSBs, γH2AX can be determined by varieties of technologies at pressent, such as gel electrophoresis (GE), immunochemiluminescence assay (ICLA) and liquid chromatography/mass spectrometry (LC/MS). γH2AX assay has already been widely used for the toxicological evaluation and clinical medicine evaluation.Figure optionsDownload as PowerPoint slide