Application of NaYF4:Yb,Er Nanoparticles as Peroxidase Mimetics in Uric Acid Detection

The poly(acrylic acid) functionalized NaYF4:Yb,Er nanoparticles (NPs) were found to possess intrinsic peroxidase-like activity, which could catalyze oxidization of 3,3′,5,5′-tetramethylbiphenyl (TMB) by H2O2 to produce a color variation. Using TMB as substrate, we systematically studied the effect of a series of conditions, such as temperature and pH, on the catalytic activity of NaYF4:Yb,Er NPs. When combined with uricase, the proposed colorimetric method could be successfully used for the determination of uric acid. Under the optimum conditions, the linear range of this method was from 1.0 × 10−5 M to 2.0 × 10−4 M with the detection limit down to 5.3 × 10−6 M. Moreover, the developed method was applied to detect uric acid in serum samples and the results were in good agreement with those reported by the hospital.

Due to the high affinity of uricase, the colorimetric method showed high selectivity toward uric acid. The interference test demonstrated that the absorbance was negligible in the presence of 5 mM creatinine, ascorbic acid, glucose, cholesterol, triglyceride, or urea under the experiment conditions.Figure optionsDownload as PowerPoint slide