Recent Progress of Colorimetric Assays Based on Gold Nanoparticles for Biomolecules

Biochemical analysis assays based on colorimetric methods using gold nanoparticles have many advantages such as high sensitivity, good selectivity, naked-eyes readout and complex instruments free. These methods have good prospects in applications. This review mainly focuses on colorimetric assays applying gold nanoparticles for biomolecules detection.

The determination (colorimetric and fluorometric) of AChE is based on RB-AuNPs. The well-dispersed RB-AuNPs (red) are induced to aggregate (purple) via electrostatic interaction in the presence of thiocholine derived from the hydrolysis of ATC catalyzed by AChE in the CSF of transgenic mice, accompanied with the fluorescence recovery of RB (the color of the stars changed from gray to green).Figure optionsDownload as PowerPoint slide