Rapid Determination of Bitertanol Content in Banana Using Three-dimensional Fluorescence Coupled with Second-order Calibration

A novel approach was developed for the direct quantitative analysis of bitertanol in banana by the incorporation of three-dimensional fluorescence with second-order calibration methodologies based on alternating trilinear decomposition (ATLD), alternating fitting residue (AFR), and self-weighted alternating trilinear decomposition (SWATLD) algorithms, respectively. The average recoveries of bitertanol in banana extract by using ATLD, AFR and SWATLD with an estimated component number of two were 98.2% ± 2.6%, 97.6% ± 2.1%, and 91.5% ± 3.5%, respectively. Moreover, the accuracy of these three algorithms was also evaluated through elliptical joint confidence region tests (EJCR) as well as figures of merit, such as sensitivity (SEN), selectivity (SEL), and limit of detection (LOD). The results demonstrate that these algorithms have been successfully applied to determine the content of bitertanol in banana extract.