کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1182339 1491726 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Direct Electrochemistry and Electrocatalysis of Myoglobin Entrapped in Konjac Glucomannan Films
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Direct Electrochemistry and Electrocatalysis of Myoglobin Entrapped in Konjac Glucomannan Films
چکیده انگلیسی

Myoglobin (Mb) was immobilized on a glass carbon electrode (GCE) by Konjac Glucomannan (KGM) and N, N-dimethylformamide (DMF) conjugate to fabricate myoglobin modified electrode (Mb-KGM-DMF/GCE). The direct electrochemistry and electrocatalysis of myoglobin on this modified electrode have been investigated. A pair of redox peaks with formal potential of –0.38 V and Δ Ep of 70 mV were obtained on the modified electrode in PBS (pH 7.0), which resulted from the FeIII/FeII couple of heme-protein. The linear dependence of peak current on scan rate in range of 0.2–9.0 V s–1 showed a typical surface-controlled electrochemical process. The Bohr effect of redox, a shift in the formal potential as a function of pH value from 5.0 to 12.0, indicated that Mb underwent a proton-coupled electron transfer process. Moreover, the obvious catalytic reduction of oxygen in solution and hydrogen peroxide by Mb was realized on Mb-KGM-DMF/GCE. The catalytic current increased linearly with the concentration of H2O2 in the range of 4.70–75.0 μ M. And the apparent Michaelis-Menten constant Km was calculated to be 80.8 μM.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Analytical Chemistry - Volume 34, Issue 12, December 2006, Pages 1683-1687