کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1182396 1491664 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fluorescence Resonance Energy Transfer Between Quantum Dots of CdSe and CdTe and Its Application for Determination of Serum Prostate-Specific Antigen
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Fluorescence Resonance Energy Transfer Between Quantum Dots of CdSe and CdTe and Its Application for Determination of Serum Prostate-Specific Antigen
چکیده انگلیسی

Fluorescence resonance energy transfer (FRET) from CdSe quantum dots to CdTe quantum dots was studied and used to determine the serum prostate-specific antigen (PSA). The effective energy transfer occurred between CdSe and CdTe in Tris-HCl buffer solution at pH 8.0, at which the CdTe fluorescence intensity increased greatly. When prostate-specific antigen (PSA) was added, the fluorescence intensity of CdSe-CdTe system decreased and the fluorescence was finally quenched because of the specific immunological reaction between the PSA antigen and the CdTe-labelled PSA antibody. There was a linear relationship between the concentrations of the PSA antigen and the fluorescence-quenching intensities. Then a new determination strategy of PSA was built by using the CdSe-CdTe system. Under optimum conditions, the linear range of PSA antigen was 2.8–10 μg L−1, with a correlation coefficient of r = 0.9992 and a detected limit of 1.5×10−2μg L−1 (n = 11).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Analytical Chemistry - Volume 40, Issue 2, February 2012, Pages 224-229