کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1182396 | 1491664 | 2012 | 6 صفحه PDF | دانلود رایگان |

Fluorescence resonance energy transfer (FRET) from CdSe quantum dots to CdTe quantum dots was studied and used to determine the serum prostate-specific antigen (PSA). The effective energy transfer occurred between CdSe and CdTe in Tris-HCl buffer solution at pH 8.0, at which the CdTe fluorescence intensity increased greatly. When prostate-specific antigen (PSA) was added, the fluorescence intensity of CdSe-CdTe system decreased and the fluorescence was finally quenched because of the specific immunological reaction between the PSA antigen and the CdTe-labelled PSA antibody. There was a linear relationship between the concentrations of the PSA antigen and the fluorescence-quenching intensities. Then a new determination strategy of PSA was built by using the CdSe-CdTe system. Under optimum conditions, the linear range of PSA antigen was 2.8–10 μg L−1, with a correlation coefficient of r = 0.9992 and a detected limit of 1.5×10−2μg L−1 (n = 11).
Journal: Chinese Journal of Analytical Chemistry - Volume 40, Issue 2, February 2012, Pages 224-229