کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1182477 1491722 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of the Effectiveness of Superoxide Dismutases Mediated by Liposomes for Reducing Intracellular Oxidative Stress Using Microchip Electrophoresis with Laser-Induced Fluorescence
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Evaluation of the Effectiveness of Superoxide Dismutases Mediated by Liposomes for Reducing Intracellular Oxidative Stress Using Microchip Electrophoresis with Laser-Induced Fluorescence
چکیده انگلیسی

Superoxide dismutases (SOD) can be used as anti-oxidization drugs and can eliminate superoxide radicals to protect cells from damage induced by free radicals. However, because of their large molecular weight and impermeability to cell membrane, their drug effectiveness is greatly reduced. Fluorescent microscopy images of cells demonstrated that nano-liposome could transfer SOD into cells. The anti-oxidization action of liposome-mediated SOD has been evaluated by the determination of the fluorescent intensity of intracellular reactive oxygen species (ROS) and reduced glutathione (GSH) in single cells by microchip electrophoresis with laser-induced fluorescence. It has been observed that intracellular ROS decreased and GSH increased after incubation with SOD encapsulated liposomes for 2 hours when compared with direct incubating cells with SOD. The results demonstrate that the drug effectiveness of SOD for reducing intracellular oxidative stress is significantly improved by the delivery of SOD mediated by liposomes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Analytical Chemistry - Volume 35, Issue 4, April 2007, Pages 469-473