کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1182479 1491722 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of Free Fatty Acids by High Performance Liquid Chromatography with Fluorescence Detection and Identification with Mass Spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of Free Fatty Acids by High Performance Liquid Chromatography with Fluorescence Detection and Identification with Mass Spectrometry
چکیده انگلیسی

On a reversed-phase Eclipse XDB-C8 column, 20 fatty acids were derivatized using 2-(2-(anthracen-10-yl)-1H-naphtho [2,3-d]imidazol-1-yl) ethyl-p-toluenesulfonate (ANITS) as the pre-column derivatization reagent and were separated by high performance liquid chromatography (HPLC) in conjunction with a gradient elution with fluorescence detection and mass spectrometric identification. The reagent was reacted with fatty acids in the presence of K2CO3 catalyst at 90°C min in N,N-dimethylformamide solvent. The fluorescence excitation and emission wavelength of the derivatives were λex = 250 nm and λem = 512 nm, respectively. The identification of fatty acid derivatives was performed by online MS/APCI (atmospheric pressure chemical ionization source) in positive model detection. All linear correlation coefficients for fatty acid derivatives are over 0.9999, and the detection limits, at a signal-to-noise rate of 3:1, are 24.8-98.8 fmol for labeled fatty acids. The established method is sensitive and reproducible for the determination of free fatty acids from real samples with satisfactory results.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Analytical Chemistry - Volume 35, Issue 4, April 2007, Pages 489-494