کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1182500 1491630 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantitative Analysis of Targeted Proteins in Complex Sample Using Novel Data Independent Acquisition
ترجمه فارسی عنوان
تجزیه و تحلیل کمی از پروتئین های هدفمند در نمونه های پیچیده با استفاده از اطلاعات نوین مستقل تهیه
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی

Data Independent Acquisition (DIA) is a novel mass spectrometry (MS) scan mode for quantitative proteomics, acquiring all precursors as well as fragments without losing the low abundant ions. This method overcome the limitation of product ion quantification by high-resolution MS. We developed three DIA methods on Q-qIT-OT Tribrid MS, classic DIA, novel Wide isolation window SIM scan DIA (WiSIM-DIA) and Full MS-DIA. WiSIM-DIA utilizes ultra-high resolution SIM scan for quantification, complementary with classic DIA. Quantitative analysis of 10 low abundant peptides spiked in Hela digest was performed by these three methods for linearity, reproducibility and sensitivity evaluation. The results showed that the LOQs reached amol level (14−435 amol) with good linearity and effective MS/MS confirmation. The isolation window of Full MS-DIA was as low as 3 amu, and the data can be directly used for database searching. This method realized the integration of Data dependent acquisition (DDA) and DIA, and thus overcoming the limitation of using spectra library.

Graphical AbstractThree DIA methods were developed on Q-qIT-OT Tribrid MS, including classic DIA, novel WiSIM-DIA and Full MS-DIA. WiSIM-DIA utilizes ultra-high resolution SIM scan for quantification, complementary with classic DIA. The isolation window of Full MS-DIA was as low as 3 amu, and the data could be directly used for database searching. This method realized the integration of DDA and DIA, and thus overcoming the limitation of using spectra library.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Analytical Chemistry - Volume 42, Issue 12, December 2014, Pages 1750–1758
نویسندگان
, , , ,