Progress in Mass Spectrometry Acquisition Approach for Quantitative Proteomics

As the major approach for quantitative proteomics, classic quantitative mass spectrometry (MS) faces new challenges, such as interferences from complex matrices and limits on analytical throughput. Recent progresses in MS technologies, including development of synchronous precursor selection (SPS), mass defect isobaric labeling, parallel reaction monitoring (PRM), multiplexing acquisition (MSX), and various novel data-independent acquisition (DIA) strategies, provide viable solutions for problems in relative and absolute quantification in proteomics. This review analyzed the current bottlenecks in the field of quantitative proteomics, summarized the most recent advances in quantitative MS acquisition, and highlighted the characteristics and the advantages of these new techniques in quantitative proteomics.

Graphical AbstractRecent progresses in MS technologies, including development of synchronous precursor selection (SPS), mass defect isobaric labeling, parallel reaction monitoring (PRM), multiplexing acquisition (MSX), and various novel data-independent acquisition (DIA) strategies, provide viable solutions for issues and limitations in relative and absolute quantification in proteomics.Figure optionsDownload as PowerPoint slide