کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1182535 1491613 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analysis of Urine Peptidome and Post-translation Modifications by Nano Liquid Chromatography-High Resolution Tandem Mass Spectrometry
ترجمه فارسی عنوان
تجزیه و تحلیل تغییرات پپتید ادرار و تغییرات پس از ترجمه توسط کروماتوگرافی مایع نانو
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی

Proteomics is an emerging field branching from proteomics that targets endogenous peptides of a whole organism or a subsystem. Many peptides in body fluids including urine are biomarkers with higher clinical sensitivity and specificity. In this study, we separated and enriched these peptides in human urine by LaPO4-graphene oxide composite nanomaterial, then identified and analyzed them by nano liquid chromatography-high resolution tandem mass spectrometry. In single urine sample, we identified 790 peptides which belonged to 123 proteins and some post-translation modifications including oxidation, phosphorylation, deamidated etc. There existed a series of peptide ladders in urine peptidomic. Finally, the string analysis at protein level revealed strong interaction among these proteins to which the identified peptides belong. This method can provide support for finding the biomarkers of disease in urine.

Here, we separated and enriched the low abundance peptides in human urine by LaGM composites, then identified and analyzed by nanoLC-MS/MS. We identified 790 peptides arised from 123 proteins and PTMs. Finally, the analysis at protein level revealed strong interaction among these proteins. This method can provide support for urine peptidomics analysis.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Analytical Chemistry - Volume 44, Issue 5, May 2016, Pages 716–722
نویسندگان
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