کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1182556 | 1491725 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A Novel Desalting Method on Target for MALDI-TOF-MS Analysis Based on Fe3O4/TiO2 Nanoparticles
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A novel method for desalting protein directly on MALDI-TOF-MS target has been established based on the absorption of peptides with Fe3O4/TiO2 nanoparticles. After optimization of experimental conditions, such as the amounts of nanoparticles and washing solutions, 100 fmol myoglobin digested by trypsin in 10 M urea solution could be identified successfully, and reliable results could also be obtained for 10 fmol myoglobin in 3 M urea solution. The experimental results show that the method developed can be easily and effectively used for the protein identification in high-salt buffer and has great potential for the large scale proteomics studies in future.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Analytical Chemistry - Volume 35, Issue 1, January 2007, Pages 1-6
Journal: Chinese Journal of Analytical Chemistry - Volume 35, Issue 1, January 2007, Pages 1-6