Enantioseparation of Seven Amino Alcohols on Teicoplanin Chiral Column

Under the polar organic mode, seven amino alcohols, propranolol, metoprolol, bisoprololfumarate, atenolol, salbutamol, isoprenaline, metoprolol and labetalol, were enantioseparated on teicoplanin chiral stationary phase (CSP), using methanol as mobile phase and acetic acid (HOAc) and triethylamine (TEA) as mobile phase additives. When the proportion of HOAc and TEA was 1:1, the retention factors and the resolutions of seven amino alcohols decreased with the increase of HOAc/TEA concentration, and the separation factors being kept constant. The excessive acid increased the polarity of mobile phase, and therefore the eluting ability was increased. Without HOAc additive, the retention factors of the amino alcohols decreased with the increase of the amount of TEA, with the separation factors being kept constant except labetalol. The polarity of mobile phase increased with the increasing of TEA concentration; therefore, the hydrogen bonding between solutes and CSPs decreased. All the results showed that the proportion of HOAc and TEA did not change the essentiality of chiral separation.