کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1182877 | 1491703 | 2008 | 5 صفحه PDF | دانلود رایگان |

The optimal conditions were established for the extraction of paralytic shellfish poisoning toxins from gonad of Chlamys nobilis by hydrochloric acid (HCl) and acetic acid (HAc) in the concentration range of 0.04–1.0 M. A 10-g portion of gonad of Chlamys nobilis was extracted using 10 ml acetic acid and hydrochloric acid by boiling for 5 min in a 50-ml beaker. Meanwhile, a portion of gonad of Chlamys nobilis was extracted using 0.3 M HAc and 0.2 M HCl by ultrasonic bath treatment for a total period of 5–30 min. The raw extract was centrifuged, and the pH of supernatant was adjusted from 2.0 to 4.0 using 0.1 M NaOH or 5 M HCl. After passing through a Millipore ultrafiltration membrane (10000 MW cut-off), ultrafiltrate was then analyzed by HPLC. The results showed that HCl in the concentration range of 0.25–1.0 M caused a significant decrease of C1,2 and GTX5 and the concomitant increase of GTX2,3. However, the amount of the three unstable toxins did not show obvious changes in the extraction with 0.04–0.25 M HCl and 0.04–1.0 M HAc. The complete image of the results in this study showed that HAc in the concentrations up to 1.0 M and HCl up to 0.25 M can be used as extraction solvent for PSP toxins without changing the toxin profile. Under the same concentration of HAc (0.3 M) and HCl (0.2 M), the content of C1 in the ultrasonic extraction was obviously lower than that in the boiling one, whereas C2 in the former showed slightly higher than that in the latter.
Journal: Chinese Journal of Analytical Chemistry - Volume 36, Issue 11, November 2008, Pages 1460-1464