کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1182931 1491688 2010 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A Tandem Mass Spectrometry Acquisition Strategy Based on Exclusion of Precursor Ions
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A Tandem Mass Spectrometry Acquisition Strategy Based on Exclusion of Precursor Ions
چکیده انگلیسی

Because of the complexity of proteome samples, comprehensive analysis to characterize all proteins is still not possible with present methodologies. It has been shown that replicate runs could increase the number of identified proteins. However, the redundancy of protein identifications was high. High-abundance peptides tended to be repeatedly analyzed in different runs. To reduce the redundancy and improve the efficiency of identification, the tandem mass spectrometry (MS/MS) acquisition method of linear ion trap-Fourier transform ion cyclotron resonance mass spectrometry was studied, and an acquisition strategy based on exclusion of precursor ions was developed. It proved that the strategy could extremely reduce the redundancy of MS/MS acquisition and improve the efficiency of protein identifications.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Analytical Chemistry - Volume 38, Issue 2, February 2010, Pages 241-244