کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1182988 1491806 2014 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Worm peptidomics
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Worm peptidomics
چکیده انگلیسی


• 3 families of neuropeptides occur in nematodes: FLP, NLP and INS peptides.
• Peptidomes of 3 nematodes (Caenorhabditis elegans, Caenorhabditis briggsae and Ascaris suum) have been explored.
• C. elegans processing enzymes were characterized by differential peptidomics.

Bioactive peptides are present in all metazoan species where they orchestrate diverse functions. In the last decade, high-throughput approaches based on mass spectrometry helped the identification of endogenously occurring peptides in different species. We here review biochemical strategies to obtain sequence information of natural (non-tryptic) peptides in Caenorhabditis elegans and in the related nematodes Caenorhabditis briggsae and Ascaris suum with particular attention for sample preparation and methodology. In addition, we describe seven new C. elegans neuropeptides that we recently discovered by sequencing additional peptides. Finally, we explain how differential peptidomics approaches were used to characterize key neuropeptide processing enzymes.

Immunostaining of the anterior region of Caenorhabditis elegans with anti-RFamide antibody with overlay of MS/MS spectrum from the first novel FLP peptide (from the FLP-24 precursor) as found by 2D-nanoLC Q-TOF MS/MS (Husson et al., 2005, Biochem. Biophys. Res. Commun. 335, 76–86).Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: EuPA Open Proteomics - Volume 3, June 2014, Pages 280–290
نویسندگان
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