Determination of Trace Bisphenol A in Water Using Three-phase Hollow Fiber Liquid-phase Microextraction Coupled with High Performance Liquid Chromatography

A method for the determination of trace bisphenol A (BPA) in water was developed by high performance liquid chromatography (HPLC) using three-phase hollow fiber liquid-phase microextraction (HF-LPME) as sample preparation technique. The system of three-phase hollow fiber liquid-phase microextraction was designed. The optimized microextraction conditions were as follows: n-octanol was used as the extraction solvent, 0.09 M NaOH solution was used as acceptor phase, the pH value of sample solutions was 4.0, 30 g L−1 NaCl was solved in sample solutions, the stirring rate was 900 rpm and the extraction time was 60 min. The analysis of BPA was finished with injecting 20 μL acceptor phase by HPLC. Under the optimal microextraction conditions, the linear range for BPA was 0.5–200 μg L−1 (r > 0.999), the detection limit (S/N = 3) of BPA was 0.2 μg L−1, the enrichment factor was 241, and the relative standard deviations (RSD) were lower than 3.2%. This method was applied to the determination of the BPA in environmental water samples. The average spiked recoveries were within the range of 92.8%–101.9% (n = 3). The developed method was simple and environmentally friendly for the determination of BPA in water.