Application and Research Development of Surface Plasmon Resonance-based Immunosensors for Protein Detection

Surface plasmon resonance (SPR) technique, which was initially developed as a novel detection strategy in the 1990s, can be applied for monitoring of interaction between ligand and analyte on a sensor chip. Thus, it has been largely demanded in the field of life science, medical testing, drug screening, food and environmental monitoring, and so on. As well known, the determination of protein concentration is of particular interest. Combination of SPR and immunoassay has been used for protein detection, with specific reaction of antigen and antibody. Applications of protein SPR immunosensors were summarized in this review, including food analysis and clinical diagnosis. Meanwhile, the authors also tried to review recent popular studies and their progress in this field.