کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1183319 1491721 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantification of Polymerase Chain Reaction Designs to Analyze the Ancient Deoxyriboncleic Acid Samples Excavated in Xiaohe Cemetery, Xinjiang, China
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Quantification of Polymerase Chain Reaction Designs to Analyze the Ancient Deoxyriboncleic Acid Samples Excavated in Xiaohe Cemetery, Xinjiang, China
چکیده انگلیسی

Human DNA quantification has become an essential means to evaluate the preservation of ancient samples and to ensure the authentication of the results in ancient DNA research. SYBR green I real-time polymerase chain reaction (PCR) was used to quantify five samples, which were excavated in Xiaohe Cemetery, China. Three different fragment sizes (138 bp, 209 bp and 363 bp) within the mitochondrial DNA hypervariable region I (HVR-I) and the AMG gene (121/115 bp) of the nuclear DNA were analyzed. The quantification results of mitochondrial DNA showed an inverse correlation between amplicon length and amplification efficiency, and only the copy numbers of 138 bp DNA were all higher than 150 copies μ1−1. Although majority of the copy numbers of AMG gene were lower than 30 copies μl−1, STR and SNP of nuclear DNA could also be analyzed. This study also showed that tooth samples had more DNA copies than bone samples.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Analytical Chemistry - Volume 35, Issue 5, May 2007, Pages 658-662